Introduction to Growth Hormone Releasing Hormone (GHRH) Detection Kit - Huaqiang

Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

EL-C1600N100013-B

Growth hormone releasing hormone (GHRH) test kit

Basic Information

What is the growth hormone releasing hormone tumor? Clinically, somatostatin is a 14 amino acid polypeptide originally isolated from the rat hypothalamus in 1968 and found to inhibit the release of growth hormone. It is named as a growth hormone release inhibitor. Thalamic pancreatic D cells have somatostatin in the gastroduodenum and small intestine and can widely inhibit the release of various peptides. Therefore, this hormone can not only inhibit endocrine and exocrine but also inhibit intestinal peristalsis and gallbladder contraction. In order to inhibit hormones, Ganda and Larsson first independently described somatostatin tumors in their own reports in 1977.

Growth hormone releasing hormone (GHRH) test kit

Color and color

Color development

Color development is the last incubation step in an ELISA assay where the enzyme catalyzes a colorless substrate to produce a colored product. The temperature and time of the reaction are still factors that affect color development. Properly increasing the temperature helps to accelerate color development. The negative holes remain colorless for a certain period of time, while the positive holes are colored for a prolonged period of time. In the quantitative test, the reaction temperature and time after the addition of the substrate should be determined according to the regulations. The ELISA coloration result is best carried out by a microplate reader, which can reduce the experimental error and improve the analysis accuracy of the critical value sample. Try to avoid direct judgment of the results by the naked eye, because the visual differences of different individuals. It should be noted that when the developer is added, the developer is kept out of the flow. Avoid adding more bubbles when adding stop solution, otherwise the probability of false positive results may increase.

Colorimetric

The colorimetric method includes two methods: a visual method and an enzyme standard colorimetric method. The visual method is simple and straightforward, but for the same specimen, the operator's difference sometimes has different results and has certain subjectivity. Colorimetric results are usually expressed in terms of optical density and are now expressed as absorbance as specified. The microplate reader should not be placed under sunlight or strong light. The suitable room temperature during operation is between 15 °C and 30 °C. Preheat the instrument for 15~30 minutes before use, so that the measured results are more accurate. Before colorimetry, clean the liquid attached to the bottom of the plate with a clean absorbent paper, and then place the plate correctly on the colorimetric frame of the enzyme-based colorimeter. In summary, high-quality reagents, good instrumentation and correct operation are necessary conditions to ensure accurate and reliable ELISA results. In the actual application process, the operator must be proficient in basic operational skills, conduct strict and careful verification of each link, try to avoid the occurrence of false positive and false negative reactions, ensure the test results are true and reliable, and provide reliable diagnosis for clinical diagnosis and disease prevention. The theoretical basis.

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